A Yegorova, I Leonenko, Yu Scrypynets, G Maltsev, V Antonovich
Methods and applications in fluorescence 2016 Sep 14Under physiological conditions, in vitro interaction between the bio-active substance 6-(2-morpholin-4-yl-ethyl)-6H-indolo[2,3-b]quinoxaline hydrochloride (MIQ) and human serum albumin (HSA) was investigated at an excitation wavelength 260 nm and at different temperatures (298 K, 308 K and 313 K) by fluorescence emission spectroscopy. From spectral analysis, MIQ showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant is estimated asK A = 2.55 × 10-4 l · mol-1 at 298 K. Based on the thermodynamic parameters evaluated from the van 't Hoff equation, the enthalpy change (ΔH°) and entropy change (ΔS°) were derived to be negative values. A value of 2.37 nm for the average distance r between MIQ (acceptor) and tryptophan residues of HSA (donor) was derived from the fluorescence resonance energy transfer. UV/vis absorption spectra were used to confirm the quenching mechanism.
A Yegorova, I Leonenko, Yu Scrypynets, G Maltsev, V Antonovich. Study on the interaction of 6-(2-morpholin-4-yl-ethyl)-6H-indolo [2,3-b]quinoxaline hydrochloride with human serum albumin by fluorescence spectroscopy. Methods and applications in fluorescence. 2016 Sep 14;4(3):034012
PMID: 28355158
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