Correlation Engine 2.0
Clear Search sequence regions

  • basta (3)
  • cell (6)
  • chitin (5)
  • endows (1)
  • human (2)
  • products (1)
  • sponges (1)
  • stromal cells (2)
  • Sizes of these terms reflect their relevance to your search.

    The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce "ready-to-use" tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications. Copyright © 2017 Elsevier B.V. All rights reserved.


    Vitalii V Mutsenko, Oleksandr Gryshkov, Lothar Lauterboeck, Olena Rogulska, Dmitriy N Tarusin, Vasilii V Bazhenov, Kathleen Schütz, Sophie Brüggemeier, Elke Gossla, Ashwini R Akkineni, Heike Meißner, Anja Lode, Stephan Meschke, Jane Fromont, Allison L Stelling, Konstantin R Tabachnik, Michael Gelinsky, Sergey Nikulin, Sergey Rodin, Alexander G Tonevitsky, Alexander Y Petrenko, Birgit Glasmacher, Peter J Schupp, Hermann Ehrlich. Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells: Biocompatibility and cryopreservation. International journal of biological macromolecules. 2017 Nov;104(Pt B):1955-1965

    PMID: 28365291

    View Full Text