REEP6 mediates trafficking of a subset of Clathrin-coated vesicles and is critical for rod photoreceptor function and survival.

Human molecular genetics 2017 Jun 15

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In retinal photoreceptors, vectorial transport of cargo is critical for transduction of visual signals, and defects in intracellular trafficking can lead to photoreceptor degeneration and vision impairment. Molecular signatures associated with routing of transport vesicles in photoreceptors are poorly understood. We previously reported the identification of a novel rod photoreceptor specific isoform of Receptor Expression Enhancing Protein (REEP) 6, which belongs to a family of proteins involved in intracellular transport of receptors to the plasma membrane. Here we show that loss of REEP6 in mice (Reep6-/-) results in progressive retinal degeneration. Rod photoreceptor dysfunction is observed in Reep6-/- mice as early as one month of age and associated with aberrant accumulation of vacuole-like structures at the apical inner segment and reduction in selected rod phototransduction proteins. We demonstrate that REEP6 is detected in a subset of Clathrin-coated vesicles and interacts with the t-SNARE, Syntaxin3. In concordance with the rod degeneration phenotype in Reep6-/- mice, whole exome sequencing identified homozygous REEP6-E75K mutation in two retinitis pigmentosa families of different ethnicities. Our studies suggest a critical function of REEP6 in trafficking of cargo via a subset of Clathrin-coated vesicles to selected membrane sites in retinal rod photoreceptors. © The Author 2017. Published by Oxford University Press.

Citation

Shobi Veleri, Jacob Nellissery, Bibhudatta Mishra, Souparnika H Manjunath, Matthew J Brooks, Lijin Dong, Kunio Nagashima, Haohua Qian, Chun Gao, Yuri V Sergeev, Xiu-Feng Huang, Jia Qu, Fan Lu, Artur V Cideciyan, Tiansen Li, Zi-Bing Jin, Robert N Fariss, Rinki Ratnapriya, Samuel G Jacobson, Anand Swaroop. REEP6 mediates trafficking of a subset of Clathrin-coated vesicles and is critical for rod photoreceptor function and survival. Human molecular genetics. 2017 Jun 15;26(12):2218-2230