Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D.
Christopher G F Graf, Christian Schulz, Marina Schmälzlein, Christian Heinlein, Manuel Mönnich, Lukas Perkams, Markus Püttner, Irene Boos, Markus Hessefort, Jose Nelson Lombana Sanchez, Michael Weyand, Clemens Steegborn, Bernadette Breiden, Kerstin Ross, Günter Schwarzmann, Konrad Sandhoff, Carlo Unverzagt
Angewandte Chemie (International ed. in English) 2017 May 02The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid-phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid-binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N-glycan. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Citation
Christopher G F Graf, Christian Schulz, Marina Schmälzlein, Christian Heinlein, Manuel Mönnich, Lukas Perkams, Markus Püttner, Irene Boos, Markus Hessefort, Jose Nelson Lombana Sanchez, Michael Weyand, Clemens Steegborn, Bernadette Breiden, Kerstin Ross, Günter Schwarzmann, Konrad Sandhoff, Carlo Unverzagt. Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D. Angewandte Chemie (International ed. in English). 2017 May 02;56(19):5252-5257
PMID: 28378443
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