Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretion chaperone VirE1. It was also biologically functional in complementing virE2 null strains transforming Arabidopsis thaliana roots and Nicotiana tabacum leaves. In vitro experiments demonstrated a two-color fluorescent complex using VirE2-TC/ReAsH and Alexa Fluor 488 labeled ssDNA. In vivo, fluorescent VirE2-TC/ReAsH was detected in bacteria and in plant cells at time frames relevant to transformation.


Noga Yaakov, Yoav Barak, Idan Pereman, Peter J Christie, Michael Elbaum. Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens. PloS one. 2017;12(4):e0175273

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 28403156

View Full Text