A yeast growth assay to characterize plant poly(ADP-ribose) polymerase (PARP) proteins and inhibitors.

Dagmar Rissel, Peter Paul Heym, Edgar Peiter

Analytical biochemistry 2017 Jun 15

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Poly(ADP-ribose) polymerases (PARPs) have been implicated in responses of plants to DNA damage and numerous stresses, whereby the mechanistic basis of the interference is often unclear. Therefore, the identification of specific inhibitors and potential interactors of plant PARPs is desirable. For this purpose, we established an assay based on heterologous expression of PARP genes from the model plant Arabidopsis thaliana in yeast. Expression of AtPARPs caused an inhibition of yeast growth to different extent, which was alleviated by inhibitors targeted at human PARPs. This assay provides a fast and simple means to identify target proteins and pharmacological inhibitors of AtPARP1. Copyright © 2017 Elsevier Inc. All rights reserved.

Citation

Dagmar Rissel, Peter Paul Heym, Edgar Peiter. A yeast growth assay to characterize plant poly(ADP-ribose) polymerase (PARP) proteins and inhibitors. Analytical biochemistry. 2017 Jun 15;527:20-23