Experimental Systems to Study Yeast Pexophagy.

Shun-Ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, Yukio Fujiki

Methods in molecular biology (Clifton, N.J.) 2017

filter terms:

Peroxisome abundance is tightly regulated according to the physiological contexts, through regulations of both proliferation and degradation of the organelles. Here, we describe detailed methods to analyze processes for autophagic degradation of peroxisomes, termed pexophagy, in yeast organisms. The assay systems include a method for biochemical detection of pexophagy completion, and one for microscopic visualization of specialized membrane structures acting in pexophagy. As a model yeast organism utilized in studies of pexophagy, the methylotrophic yeast Komagataella phaffii (Pichia pastoris) is referred to in this chapter and related information on the studies with baker's yeast (Saccharomyces cerevisiae) is also included. The described techniques facilitate elucidation of molecular machineries for pexophagy and understanding of peroxisome-selective autophagic pathways.

Citation

Shun-Ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, Yukio Fujiki. Experimental Systems to Study Yeast Pexophagy. Methods in molecular biology (Clifton, N.J.). 2017;1595:249-255