Ultra-sensitive detection of ibuprofen (IBP) by electrochemical aptasensor using the dendrimer-quantum dot (Den-QD) bioconjugate as an immobilization platform with special features.

Mahmoud Roushani, Faezeh Shahdost-Fard

Materials science & engineering. C, Materials for biological applications 2017 Jun 01

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This study describes a high-performance electrochemical aptasensor which is employed to detect Ibuprofen (IBP) as a painkiller drug by using a novel platform as an integrated sensing interface. In order to make the aptasensor, the Den-QD bioconjugate was immobilized on the surface of a GC electrode and followed the Apt was incubated on this surface. The incubation of the IBP on the aptasensor surface and the formation of the Apt/IBP complex, led to a hindered electron transfer reaction on the sensing surface, which decreased the peak current of the redox probe. Under the optimum condition, the assay had two dynamic ranges with a detection limit down to 333fM. The developed aptasensor reliably detects IBP in a real sample. Our results demonstrated that the proposed strategy has many advantages and the Den-QD bioconjugate may become a promising nanocomposite for the electrochemical sensing applications. Copyright © 2017. Published by Elsevier B.V.

Citation

Mahmoud Roushani, Faezeh Shahdost-Fard. Ultra-sensitive detection of ibuprofen (IBP) by electrochemical aptasensor using the dendrimer-quantum dot (Den-QD) bioconjugate as an immobilization platform with special features. Materials science & engineering. C, Materials for biological applications. 2017 Jun 01;75:1091-1096