Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls.

Daniel J Cosgrove, Nathan K Hepler, Edward R Wagner, Daniel M Durachko

Methods in molecular biology (Clifton, N.J.) 2017

filter terms:

Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here, we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

Citation

Daniel J Cosgrove, Nathan K Hepler, Edward R Wagner, Daniel M Durachko. Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls. Methods in molecular biology (Clifton, N.J.). 2017;1588:157-165