Appendix C: Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device.

This chapter introduces a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual microdroplets manipulated on the microfluidic device were used as microvessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.

Citation

Jun Liu, Derek G Pyne, Mohamed Abdelgawad, Yu Sun. Appendix C: Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device. Methods in molecular biology (Clifton, N.J.). 2017;1568:309-316

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PMID: 28421507

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