Correlation Engine 2.0
Clear Search sequence regions

  • ags (1)
  • ags cells (2)
  • AMPK (7)
  • Ca2 (1)
  • calmodulin (1)
  • cell (4)
  • gastric cancer (6)
  • human (6)
  • human cells (1)
  • microrna (1)
  • Ppm1E (9)
  • shrna (1)
  • tissues (3)
  • Sizes of these terms reflect their relevance to your search.

    Activation of AMP-activated protein kinase (AMPK) is a valuable anti-cancer strategy. In the current study, we tested expression and potential function of Ca2+/calmodulin-dependent protein kinase phosphatase (Ppm1E), an AMPKα phosphatase, in human gastric cancers. Ppm1E expression was elevated in human gastric cancer tissues (vs. normal tissues), which was correlated with AMPK (p-AMPKα, Thr-172) dephosphorylation and mTOR complex 1 (mTORC1) activation. Ppm1E upregulation, AMPK inhibition and mTORC1 activation were also observed in human gastric cancer cell lines (AGS, HGC-27, and SNU601). Intriguingly, Ppm1E knockdown by shRNA induced AMPK activation, mTORC1 inactivation, and proliferation inhibition in AGS cells. On the other hand, forced over-expression of Ppm1E induced further AMPK inhibition and mTORC1 activation to enhance AGS cell proliferation. Remarkably, microRNA-135b-5p ("miR-135b-5p"), an anti-Ppm1E microRNA, was downregulated in both human gastric cancer tissues and cells. Reversely, miR-135b-5p exogenous expression caused Ppm1E depletion, AMPK activation, and AGC cell proliferation inhibition. Together, Ppm1E upregulation in human gastric cancer is important for cell proliferation, possible via regulating AMPK-mTOR signaling.


    Min-Bin Chen, Yuan-Yuan Liu, Li-Bo Cheng, Jian-Wei Lu, Ping Zeng, Pei-Hua Lu. AMPKα phosphatase Ppm1E upregulation in human gastric cancer is required for cell proliferation. Oncotarget. 2017 May 09;8(19):31288-31296

    PMID: 28423719

    View Full Text