Correlation Engine 2.0
Clear Search sequence regions

  • GLP 1 (4)
  • Glucagon (9)
  • glucagon like (2)
  • human (3)
  • incretins (2)
  • mass (1)
  • phase (1)
  • plasma (2)
  • study samples (1)
  • Sizes of these terms reflect their relevance to your search.

    The performance of glucagon and GLP-1 immunoassays is often poor, but few sensitive LC-MS/MS methods exist as alternatives. A multiplexed LC-MS/MS method using a 2D extraction technique was developed. The method was established for the quantitation of endogenous glucagon (LLOQ: 15 pg/ml) and dosed GLP-1 (LLOQ: 25 pg/ml) in human plasma, and is the first such method avoiding immunoenrichment. Specificity of endogenous glucagon quantitation was assured using a novel approach with a supercharging mobile phase additive to access a sensitive qualifier SRM. Endogenous glucagon concentrations were within the expected range, and showed good reproducibility after extended sample storage. A cross-validation against established immunoassays using physiological study samples demonstrated some similarities between methods. The LC-MS/MS method offers a viable alternative to immunoassays for quantitation of endogenous glucagon, dosed glucagon and/or dosed GLP-1.


    James W Howard, Richard G Kay, Ben Jones, Jaimini Cegla, Tricia Tan, Steve Bloom, Colin S Creaser. Development of a UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon and dosed GLP-1 from human plasma. Bioanalysis. 2017 May;9(9):733-751

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 28488894

    View Full Text