Aptatope mapping of the binding site of a progesterone aptamer on the steroid ring structure.

In this work we report the mapping of the binding site of the only progesterone aptamer published to date, in an approach referred to as aptatope mapping. By linking the binding data obtained from microscale thermophoresis analysis to the structural differences on the ring structure of a range of steroids, we elucidated the moieties involved in aptamer-progesterone binding. This approach can be further exploited for the characterization of aptamer specificity and ultimately facilitate the development of aptamer-based assays depending on the desired specificity. Copyright © 2017 Elsevier Inc. All rights reserved.

Citation

Vasso Skouridou, Thomas Schubert, Abdulaziz S Bashammakh, Mohammad S El-Shahawi, Abdulrahman O Alyoubi, Ciara K O'Sullivan. Aptatope mapping of the binding site of a progesterone aptamer on the steroid ring structure. Analytical biochemistry. 2017 Aug 15;531:8-11

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PMID: 28499498

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