Design and synthesis of novel photoinduced electron transfer-based hybridization probes.

Chika Yokoyama, Kosuke Nakamoto, Yoshihito Ueno

Bioorganic & medicinal chemistry 2017 Jul 01

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Photoinduced electron transfer (PeT)-based hybridization probe is a linear and quencher-free oligonucleotide (ON) probe for DNA or RNA detection. In this report, we designed and synthesized novel adenosine analogues for PeT-based hybridization probe. In particular, the analogue containing a piperazinomethyl moiety showed effective quenching property under physiological conditions. When the probe containing the analogue was hybridized with a complementary DNA or RNA, the fluorescence increased 3- or 4-fold, respectively, compared to the single-stranded state. Copyright © 2017 Elsevier Ltd. All rights reserved.

Citation

Chika Yokoyama, Kosuke Nakamoto, Yoshihito Ueno. Design and synthesis of novel photoinduced electron transfer-based hybridization probes. Bioorganic & medicinal chemistry. 2017 Jul 01;25(13):3574-3582