Graphene modified titanium alloy promote the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells.

Kewen Li, Jinhong Yan, Chunhui Wang, Long Bi, Qi Zhang, Yisheng Han

Biochemical and biophysical research communications 2017 Jul 22

filter terms:

We studied the effects of graphene coating on improving the biological activity of a titanium alloy (Ti6Al4V) widely used in hip and knee joint replacements. The experiments included immunofluorescence staining for observing cellular adhesion, Cell Counting Kit-8 (CCK-8) for evaluating cellular proliferation and reverse transcription-polymerase chain reaction (RT-PCR) for detecting the differentiation of bone marrow stromal cells on different scaffolds. The results showed that G-Ti6Al4V exhibited a higher mean integrated optical density (IOD) for vinculin and resulted in a higher cell proliferation rate and higher osteoblast-specific gene transcription levels. In summary, graphene could be used as a new nanocoating material for Ti6Al4V scaffolds to enhance their surface bioactivity. Copyright © 2017 Elsevier Inc. All rights reserved.

Citation

Kewen Li, Jinhong Yan, Chunhui Wang, Long Bi, Qi Zhang, Yisheng Han. Graphene modified titanium alloy promote the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells. Biochemical and biophysical research communications. 2017 Jul 22;489(2):187-192