Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR.

To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed. PCR screening across 143 isolates demonstrated 100% specificity for hypD and siaT. These two assays were multiplexed with the recently described fucP assay for further differentiation among H. influenzae. The triplex assay provides rapid, unambiguous, sensitive and highly specific genotyping results for the simultaneous detection of hypD and siaT, including fucose-positive H. influenzae (fucP), in a single PCR.

Citation

Erin P Price, Tegan M Harris, Jessie Spargo, Elizabeth Nosworthy, Jemima Beissbarth, Anne B Chang, Heidi C Smith-Vaughan, Derek S Sarovich. Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR. Future microbiology. 2017 Jun;12:585-593

PMID: 28604066

search → result