Exploring the Bioelectrochemical Characteristics of Activated Sludge Using Cyclic Voltammetry.

Dena Z Khater, K M El-Khatib, Rabeay Y A Hassan

Applied biochemistry and biotechnology 2018 Jan

filter terms:

Due to the potential interest, bioelectrochemical responses of activated sludge using the three-electrode system are tested. From the cyclic voltammograms, the oxidation current output is increasing due to incubation time increase, whereas 5, 25 and 39.33 μA are obtained after 3, 72 and 96 h, respectively. Changing the working electrode from glassy carbon to carbon paste led to the increase in the electrochemical signal from 0.3 to be 3.72 μA. On the other hand, the use of the lipophilic redox mediator (2,6-dichlorophenolindophenol (DCIP)) amplified the oxidation current to reach 19.9 μA instead of 2.1 μA. Based on these findings, the mixed microbial community of the activated sludge is exploited as a catalyst for the bio-oxidation of the degradable organic substrates, while DCIP is used as a mobile electron carrier from the intracellular matrix of the metabolically active cells to the carbon paste electrode which served as the final electron acceptor. Therefore, the extracellular electron transfer from the formed active biofilm at the electrode surface is assisted by the existence of DCIP.

Citation

Dena Z Khater, K M El-Khatib, Rabeay Y A Hassan. Exploring the Bioelectrochemical Characteristics of Activated Sludge Using Cyclic Voltammetry. Applied biochemistry and biotechnology. 2018 Jan;184(1):92-101