Virus purification by CsCl density gradient using general centrifugation.

Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13' and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.

Citation

Tadahiro Nasukawa, Jumpei Uchiyama, Satoshi Taharaguchi, Sumire Ota, Takako Ujihara, Shigenobu Matsuzaki, Hironobu Murakami, Keijirou Mizukami, Masahiro Sakaguchi. Virus purification by CsCl density gradient using general centrifugation. Archives of virology. 2017 Nov;162(11):3523-3528

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PMID: 28785814

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