Correlation Engine 2.0
Clear Search sequence regions

  • ATPase (1)
  • brassica rapa (1)
  • cadmium (4)
  • CAX4 (1)
  • cdna libraries (1)
  • COPT5 (1)
  • gene networks (1)
  • genes (5)
  • HMA2 (1)
  • HMA3 (1)
  • HMA4 (1)
  • ion transport (1)
  • IRT1 (1)
  • MRP7 (1)
  • MTP3 (1)
  • MTPs (1)
  • pak (5)
  • plasma (1)
  • sequenced rna (1)
  • transport (3)
  • ZIP2 (1)
  • ZIP3 (1)
  • Sizes of these terms reflect their relevance to your search.

    Cadmium translocation from roots to shoots is a complex biological process that is controlled by gene regulatory networks. Pak choi exhibits wide cultivar variations in Cd accumulation. However, the molecular mechanism involved in cadmium translocation and accumulation is still unclear. To isolate differentially expressed genes (DEGs) involved in transporter-mediated regulatory mechanisms of Cd translocation in two contrasting pak choi cultivars, Baiyewuyueman (B, high Cd accumulator) and Kuishan'aijiaoheiye (K, low Cd accumulator), eight cDNA libraries from the roots of two cultivars were constructed and sequenced by RNA-sequencing. A total of 244,190 unigenes were obtained. Of them, 6827 DEGs, including BCd10 vs. BCd0 (690), KCd10 vs. KCd0 (2733), KCd0 vs. BCd0 (2919), and KCd10 vs. BCd10 (3455), were identified. Regulatory roles of these DEGs were annotated and clarified through GO and KEEG enrichment analysis. Interestingly, 135 DEGs encoding ion transport (i.e. ZIPs, P1B-type ATPase and MTPs) related proteins were identified. The expression patterns of ten critical genes were validated using RT-qPCR analysis. Furthermore, a putative model of cadmium translocation regulatory network in pak choi was proposed. High Cd cultivar (Baiyewuyueman) showed higher expression levels in plasma membrane-localized transport genes (i.e., ZIP2, ZIP3, IRT1, HMA2 and HMA4) and tonoplast-localized transport genes (i.e., CAX4, HMA3, MRP7, MTP3 and COPT5) than low Cd cultivar (Kuishan'aijiaoheiye). These genes, therefore, might be involved in root-to-shoot Cd translocation in pak choi.


    Rugang Yu, Dan Li, Xueling Du, Shenglan Xia, Caifeng Liu, Gangrong Shi. Comparative transcriptome analysis reveals key cadmium transport-related genes in roots of two pak choi (Brassica rapa L. ssp. chinensis) cultivars. BMC genomics. 2017 Aug 08;18(1):587

    PMID: 28789614

    View Full Text