Role of stromal derived factor-1a (SDF-1a) for spermatogenesis of busulfan-injured rats.

SDF-1a is a member of CXC chemokine family that plays a crucial role in stem cell migration, cell apoptosis and development. The role of intra-scrotal administration of SDF-1a in spermatogenesis of busulfan-treated rats was investigated in this study. Two injections of busulfan (15mg/kg) with a 14days interval between were given intraperitoneally to male Wistar rats. Rats were then treated for seven days with 500ng/mL SDF-1a. Real-time PCR and immunohistochemistry were performed for evaluation of various cell markers for proliferation and spermatogenesis, and sperm parameters were assessed. In the SDF-1a group, there was a significant increase in testis weight, sperm count and viability. DAZL, DDX4, and TP2 showed increased expression levels in the SDF-1a group. PCNA and BrdU revealed highest expression rates in the SDF-1a group (p≤0.0001). These findings showed the protective role of SDF-1a in busulfan-induced testis injury most likely through stimulation of SSCs proliferation. Copyright © 2017 Elsevier Inc. All rights reserved.

Citation

Neda Khanlarkhani, Keywan Mortezaee, Fardin Amidi, Ebrahim Kharazinejad, Cordian Beyer, Maryam Baazm, Parichehr Pasbakhsh, Azar Pazhohan, Aligholi Sobhani, Adib Zendedel. Role of stromal derived factor-1a (SDF-1a) for spermatogenesis of busulfan-injured rats. Reproductive toxicology (Elmsford, N.Y.). 2017 Oct;73:142-148

PMID: 28818485

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