Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus.

Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.

Citation

Ekaterina K Donchenko, Evgeniya V Pechnikova, Maryia Yu Mishyna, Tatiana I Manukhova, Olga S Sokolova, Nikolai A Nikitin, Joseph G Atabekov, Olga V Karpova. Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus. PloS one. 2017;12(8):e0183824

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PMID: 28837650

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