Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

β-Dystroglycan (β-DG) is a transmembrane protein with critical roles in cell adhesion, cytoskeleton remodeling and nuclear architecture. This functional diversity is attributed to the ability of β-DG to target to, and conform specific protein assemblies at the plasma membrane (PM) and nuclear envelope (NE). Although a classical NLS and importin α/β mediated nuclear import pathway has already been described for β-DG, the intracellular trafficking route by which β-DG reaches the nucleus is unknown. In this study, we demonstrated that β-DG undergoes retrograde intracellular trafficking from the PM to the nucleus via the endosome-ER network. Furthermore, we provided evidence indicating that the translocon complex Sec61 mediates the release of β-DG from the ER membrane, making it accessible for importins and nuclear import. Finally, we show that phosphorylation of β-DG at Tyr890 is a key stimulus for β-DG nuclear translocation. Collectively our data describe the retrograde intracellular trafficking route that β-DG follows from PM to the nucleus. This dual role for a cell adhesion receptor permits the cell to functionally connect the PM with the nucleus and represents to our knowledge the first example of a cell adhesion receptor exhibiting retrograde nuclear trafficking and having dual roles in PM and NE.

Citation

Viridiana Gracida-Jiménez, Ricardo Mondragón-González, Griselda Vélez-Aguilera, Alejandra Vásquez-Limeta, Marco S Laredo-Cisneros, Juan de Dios Gómez-López, Luis Vaca, Sarah C Gourlay, Laura A Jacobs, Steve J Winder, Bulmaro Cisneros. Retrograde trafficking of β-dystroglycan from the plasma membrane to the nucleus. Scientific reports. 2017 Aug 29;7(1):9906


PMID: 28852008

View Full Text