Determination of azelastine and desmethylazelastine in human plasma by high-performance liquid chromatography.

J Pivonka, F H Segelman, C A Hartman, W E Segl, N Kucharczyk, R D Sofia

Wallace Laboratories, Division of Carter-Wallace, Inc., Cranbury, NJ 08512.

Journal of chromatography 1987 Sep 4

filter terms:

A manual-injection liquid chromatographic method using fluorescence detection permitted determination of a new antiasthmatic drug, azelastine, and its desmethyl metabolite extracted from human plasma. Reliable quantitation was achieved to at least 0.3 ng/ml for each analyte. No interference was seen in co-chromatography of sixteen other substances, which were potential co-medications (or their metabolites) as used in standard asthma or allergy treatment.

Citation

J Pivonka, F H Segelman, C A Hartman, W E Segl, N Kucharczyk, R D Sofia. Determination of azelastine and desmethylazelastine in human plasma by high-performance liquid chromatography. Journal of chromatography. 1987 Sep 4;420(1):89-98