Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers.

Mark A Zarnegar, Felicia Reinitz, Aaron M Newman, Michael F Clarke

Nucleic acids research 2017 Sep 29

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The complexity and inefficiency of chromatin immunoprecipitation strategies restrict their sensitivity and application when examining rare cell populations. We developed a new technique that replaces immunoprecipitation with a simplified chromatin fragmentation and proximity ligation step that eliminates bead purification and washing steps. We present a simple single tube proximity ligation technique, targeted chromatin ligation, that captures histone modification patterns with only 200 cells. Our technique eliminates loss of material and sensitivity due to multiple inefficient steps, while simplifying the workflow to enhance sensitivity and create the potential for novel applications. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Citation

Mark A Zarnegar, Felicia Reinitz, Aaron M Newman, Michael F Clarke. Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers. Nucleic acids research. 2017 Sep 29;45(17):e153