Interaction of Cholera Toxin B-subunit with Human T-lymphocytes.

E V Navolotskaya, V B Sadovnikov, D V Zinchenko, Y A Zolotarev, V M Lipkin, V P Zav'yalov

Biochemistry. Biokhimiia 2017 Sep

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In this work, 125I-labeled cholera toxin B-subunit (CT-B) (specific activity 98 Ci/mmol) was prepared, and its high-affinity binding to human blood T-lymphocytes (Kd = 3.3 nM) was determined. The binding of the 125I-labeled CT-B was inhibited by unlabeled interferon-α2 (IFN-α2), thymosin-α1 (TM-α1), and by the synthetic peptide LKEKK, which corresponds to sequences 16-20 of human TM-α1 and 131-135 of IFN-α2 (Ki 0.8, 1.2, and 1.6 nM, respectively), but was not inhibited by the unlabeled synthetic peptide KKEKL with inverted sequence (Ki > 1 µM). In the concentration range of 10-1000 nM, both CT-B and peptide LKEKK dose-dependently increased the activity of soluble guanylate cyclase (sGC) but did not affect the activity of membrane-bound guanylate cyclase. The KKEKL peptide tested in parallel did not affect sGC activity. Thus, the CT-B and peptide LKEKK binding to a common receptor on the surface of T-lymphocytes leads to an increase in sGC activity.

Citation

E V Navolotskaya, V B Sadovnikov, D V Zinchenko, Y A Zolotarev, V M Lipkin, V P Zav'yalov. Interaction of Cholera Toxin B-subunit with Human T-lymphocytes. Biochemistry. Biokhimiia. 2017 Sep;82(9):1036-1041