FlexED8: the first member of a fast and flexible sample-changer family for macromolecular crystallography.

Gergely Papp, Franck Felisaz, Clement Sorez, Marcos Lopez-Marrero, Robert Janocha, Babu Manjasetty, Alexandre Gobbo, Hassan Belrhali, Matthew W Bowler, Florent Cipriani

Acta crystallographica. Section D, Structural biology 2017 Oct 01

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Automated sample changers are now standard equipment for modern macromolecular crystallography synchrotron beamlines. Nevertheless, most are only compatible with a single type of sample holder and puck. Recent work aimed at reducing sample-handling efforts and crystal-alignment times at beamlines has resulted in a new generation of compact and precise sample holders for cryocrystallography: miniSPINE and NewPin [see the companion paper by Papp et al. (2017, Acta Cryst., D73, 829-840)]. With full data collection now possible within seconds at most advanced beamlines, and future fourth-generation synchrotron sources promising to extract data in a few tens of milliseconds, the time taken to mount and centre a sample is rate-limiting. In this context, a versatile and fast sample changer, FlexED8, has been developed that is compatible with the highly successful SPINE sample holder and with the miniSPINE and NewPin sample holders. Based on a six-axis industrial robot, FlexED8 is equipped with a tool changer and includes a novel open sample-storage dewar with a built-in ice-filtering system. With seven versatile puck slots, it can hold up to 112 SPINE sample holders in uni-pucks, or 252 miniSPINE or NewPin sample holders, with 36 samples per puck. Additionally, a double gripper, compatible with the SPINE sample holders and uni-pucks, allows a reduction in the sample-exchange time from 40 s, the typical time with a standard single gripper, to less than 5 s. Computer vision-based sample-transfer monitoring, sophisticated error handling and automatic error-recovery procedures ensure high reliability. The FlexED8 sample changer has been successfully tested under real conditions on a beamline.

Citation

Gergely Papp, Franck Felisaz, Clement Sorez, Marcos Lopez-Marrero, Robert Janocha, Babu Manjasetty, Alexandre Gobbo, Hassan Belrhali, Matthew W Bowler, Florent Cipriani. FlexED8: the first member of a fast and flexible sample-changer family for macromolecular crystallography. Acta crystallographica. Section D, Structural biology. 2017 Oct 01;73(Pt 10):841-851