Substrate independent Ag nanoparticles loaded hydrogel coating with regenerable bactericidal and thermo-responsive anti-bacterial properties.

We report an Ag nanoparticles (AgNPs) based substrate independent bactericidal hydrogel coating with thermo-responsive anti-bacterial property. To attach the hydrogel coating onto model substrate, ene-functionalized dopamine was firstly coated on the substrate, and then the hydrogel thin layer was formed on the surface via the UV light initiated surface cross-linking copolymerization of N-isopropylacrylamide (NIPAAm) and sodium acrylate (AANa). Then, Ag ions were adsorbed into the hydrogel layers and reduced to AgNPs by sodium borohydride. The coating showed robust bactericidal ability against Escherichia coli and Staphylococcus aureus toward both contacted bacteria and the bacteria in the surrounding. Upon a reduction of the temperature below the LCST of PNIPAAm, the improved surface hydrophilicity and swollen PNIPAAm could detach the attached dead bacteria. Meanwhile, the long-lasting and regenerable antibacterial properties could be achieved by repeatedly loading AgNPs. By precisely controlling the AgNPs loading amounts, the coating showed excellent hemocompatibility and no cytotoxity. Additionally, the coating could be applied to modify cell culture plate, since it could support cell adhesion and proliferation at 37 °C, while detach the cell by changing the temperature below lower critical solution temperature without the treatment of proteases. The study thus presents a promising way to fabricate thermo-responsive and regenerable antibacterial surfaces on diverse materials and devices for biomedical applications.

Citation

Min He, Qian Wang, Jue Zhang, Weifeng Zhao, Changsheng Zhao. Substrate independent Ag nanoparticles loaded hydrogel coating with regenerable bactericidal and thermo-responsive anti-bacterial properties. ACS applied materials & interfaces. 2017 Oct 16

PMID: 29035025

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