Isolation, purification and characterization of 5'-phosphodiesterase from Aspergillus fumigatus.

Zhiting Luo, Yingying Fan, Qiuxia Li, Bing Han, Yang Liu, Shubo Li, Hua Qiu, Zongwen Pang

PloS one 2017

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5'-Phosphodiesterase (5'-PDE) catalyzes the hydrolysis of ribonucleic acid to obtain a mixture of ribonucleotides, such as 5'-guanosine monophosphate and 5'-adenosine monophosphate. In this study, a 5'-PDE was newly isolated and purified from Aspergillus fumigatus. Following purification, this enzyme exhibited a specific activity of 1036.76 U/mg protein, a molecular weight of 9.5 kDa, and an optimal temperature and pH for enzyme activity of 60°C and 5.0, respectively. However, its activity was partially inhibited by Fe3+, Cu2+, and Zn2+, but slightly improved by the presence of K+ and Na+. Additionally, chemical-modification experiments were also applied to investigate the structural information of 5'-PDE, in which the residues containing carboxyl and imidazole groups were essential for enzyme activity based on their localization in the 5'-PDE active site. Furthermore, purified 5'-PDE could specifically catalyze the synthesis of ribonucleotides with a Vmax 0.71 mmol/mg·min and a KM of 13.60 mg/mL.

Citation

Zhiting Luo, Yingying Fan, Qiuxia Li, Bing Han, Yang Liu, Shubo Li, Hua Qiu, Zongwen Pang. Isolation, purification and characterization of 5'-phosphodiesterase from Aspergillus fumigatus. PloS one. 2017;12(10):e0186011