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Polyamines not only affect transcription and translation but also may induce a number of posttranslational modifications. The identification of polyamine-induced posttranslational modifications can be performed by 2D PAGE analyses. Here, we provide a protocol for 2D-gel electrophoresis that has been optimized for plants. The combined use of this protocol with epitope-tagged proteins expressed in plants enables the detailed analysis of posttranslational modifications induced by different polyamines in vivo.


Marta Bitrián, Antonio F Tiburcio, Rubén Alcázar. Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines. Methods in molecular biology (Clifton, N.J.). 2018;1694:337-341

PMID: 29080178

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