SFRP2/DPP4 and FMO1/LSP1 define major fibroblast populations in human skin.

Fibroblasts produce matrix, regulate inflammation, mediate reparative processes, and serve as pluripotent mesenchymal cells. Analyzing digested normal human skin by single cell RNA-seq (scRNA-seq), we explored different fibroblast populations. T-distributed stochastic neighbor embedding and clustering of scRNA-seq data from six biopsies revealed two major fibroblast populations, defined by distinct genes, including SFRP2 and FMO1, expressed exclusively by these two major fibroblast populations. Further subpopulations were defined within each of the SFRP2 and FMO1 populations, as well as five minor fibroblast populations, each expressing discrete genes: CRABP1, COL11A1, FMO2, PRG4 or C2ORF40. Immunofluorescent staining confirmed that SFRP2 and FMO1 define cell types of dramatically different morphology. SFRP2+ fibroblasts were small, elongated, and distributed between collagen bundles. FMO1+ fibroblasts were larger, and distributed in both interstitial and perivascular locations. Differential gene expression by SFRP2+, FMO1+ and COL11A1+ fibroblasts suggests roles in matrix deposition, inflammatory cell retention, and connective tissue cell differentiation, respectively. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Tracy Tabib, Christina Morse, Ting Wang, Wei Chen, Robert Lafyatis. SFRP2/DPP4 and FMO1/LSP1 define major fibroblast populations in human skin. The Journal of investigative dermatology. 2017 Oct 25

PMID: 29080679

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