Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study.

Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-α activity and polymorphism in TNFR1 could lead to gene dysfunction and male infertility. The aim of this study is to determine the association of TNFR1 36 A/G polymorphism with the idiopathic azoospermia in Iranian population. This case-control study included 108 azoospermic and 119 fertile men. This research investigated the frequency of TNFR1 36 A/G polymorphism in cases who were idiopathic azoospermic men referred to Yazd Research and Clinical Center for Infertility, Iran in comparison with controls. polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the polymorphism in both case and control groups. PCR fragments were digested by Mspa1I enzyme and products were appeared by gel electrophoresis. The abundance of A→G was calculated in the azoospermic and healthy men. According to the present study, GG and AG genotypes frequency in the azoospermic men group were higher than the control group (OR= 2.298 (1.248-4.229), p=0.007), (OR=1.47 (0.869-2.498, p=0.149). Our findings also showed that G allele frequency in azoospermic men had significant difference compared to the control group (OR=2.302 (1.580-3.355), p<0.001). It seems that the GG genotype and G allele have an association with increased risk of non-obstructive azoospermia.

Citation

Hamid Reza Ashrafzadeh, Tahere Nazari, Masoud Dehghan Tezerjani, Maryam Khademi Bami, Saeed Ghasemi-Esmailabad, Nasrin Ghasemi. Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study. International journal of reproductive biomedicine (Yazd, Iran). 2017 Aug;15(8):521-526

PMID: 29082371

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