Novel WS9326A derivatives and one novel Annimycin derivative with antimalarial activity are produced by Streptomyces asterosporus DSM 41452 and its mutant.

In this study we report that Streptomyces asterosporus DSM 41452 is a producer of new molecules related to the non-ribosomal cyclodepsipeptide WS9326A and the polyketide Annimycin. S. asterosporus DSM 41452 is shown to produce six cyclodepsipeptides and peptides, WS9326A to G. Notably, the compounds WS9326F and WS9326G have not been de-scribed before. The genome of S. asterosporus DSM 41452 was sequenced and a putative WS9326A gene cluster was iden-tified. Gene deletion experiments confirmed that this cluster is responsible for the biosynthesis of WS9326A to G. Additional-ly, a gene deletion experiment demonstrated that sas16 encoding a cytochrome P450 monooxygenase is involved in the synthesis of the novel E-2,3-dehydrotyrosine residue found in WS9326A and its derivatives. An insertion mutation within the putative Annimycin gene cluster led to the production of a new Annimycin derivative, Annimycin B, which exhibited modest inhibitory activity against Plasmodium falciparum. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Andreas Bechthold, Songya Zhang, Jing Zhu, David Zechel, Claudia Jessen-Trefzer, Richard T Eastman, Thomas Paululat. Novel WS9326A derivatives and one novel Annimycin derivative with antimalarial activity are produced by Streptomyces asterosporus DSM 41452 and its mutant. Chembiochem : a European journal of chemical biology. 2017 Nov 17

PMID: 29148157

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