Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin.

Camila Henrique, Bruna A Caetano, Thais Mitsunari, Luís Fernando Dos Santos, Roxane M F Piazza, Letícia B Rocha

Journal of microbiological methods 2018 Jan

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We standardized an immunochromatographic test (IC) for heat-labile toxin I (LT-I) detection using LT-I antibodies and a specific platform containing the apparatus for application, assembly and cutting. IC detected as little as 62.5ng/mL of purified LT-I toxin and presented 91% sensitivity, 99.5% specificity and 96.0% accuracy, thereby proving to be an excellent point-of-care test for the diagnosis of enterotoxigenic E. coli infection in low-income countries. Copyright © 2017 Elsevier B.V. All rights reserved.

Citation

Camila Henrique, Bruna A Caetano, Thais Mitsunari, Luís Fernando Dos Santos, Roxane M F Piazza, Letícia B Rocha. Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin. Journal of microbiological methods. 2018 Jan;144:125-127