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    Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.


    Francesco Boin, Maria Letizia Giardino Torchia, Ivan Borrello, Kimberly A Noonan, Matthew Neil, Mark J Soloski, Raffaello Cimbro. Flow cytometric discrimination of seven lineage markers by using two fluorochromes. PloS one. 2017;12(11):e0188916

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    PMID: 29190813

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