Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.

Citation

Marcello Clerici, Marco Faini, Lena M Muckenfuss, Ruedi Aebersold, Martin Jinek. Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex. Nature structural & molecular biology. 2018 Feb;25(2):135-138

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PMID: 29358758

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