Septins are involved at the early stages of macroautophagy in S. cerevisiae.

Gaurav Barve, Shreyas Sridhar, Amol Aher, Mayurbhai H Sahani, Sarika Chinchwadkar, Sunaina Singh, Lakshmeesha K N, Michael A McMurray, Ravi Manjithaya

Journal of cell science 2018 Feb 22

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Autophagy is a conserved cellular degradation pathway wherein double-membrane vesicles called autophagosomes capture long-lived proteins, and damaged or superfluous organelles, and deliver them to the lysosome for degradation. Septins are conserved GTP-binding proteins involved in many cellular processes, including phagocytosis and the autophagy of intracellular bacteria, but no role in general autophagy was known. In budding yeast, septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants. Upon autophagy induction, pre-assembled septin complexes relocalized to the pre-autophagosomal structure (PAS) where they formed non-canonical septin rings at PAS. Septins also colocalized with autophagosomes, where they physically interacted with the autophagy proteins Atg8 and Atg9. When autophagosome degradation was blocked in septin-mutant cells, fewer autophagic structures accumulated, and an autophagy mutant defective in early stages of autophagosome biogenesis (atg1Δ), displayed decreased septin localization to the PAS. Our findings support a role for septins in the early stages of budding yeast autophagy, during autophagosome formation.This article has an associated First Person interview with the first author of the paper. © 2018. Published by The Company of Biologists Ltd.

Citation

Gaurav Barve, Shreyas Sridhar, Amol Aher, Mayurbhai H Sahani, Sarika Chinchwadkar, Sunaina Singh, Lakshmeesha K N, Michael A McMurray, Ravi Manjithaya. Septins are involved at the early stages of macroautophagy in S. cerevisiae. Journal of cell science. 2018 Feb 22;131(4)