Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

We generated a knockout mouse for the neuronal-specific β-tubulin isoform Tubb3 to investigate its role in nervous system formation and maintenance. Tubb3-/- mice have no detectable neurobehavioral or neuropathological deficits, and upregulation of mRNA and protein of the remaining β-tubulin isotypes results in equivalent total β-tubulin levels in Tubb3-/- and wild-type mice. Despite similar levels of total β-tubulin, adult dorsal root ganglia lacking TUBB3 have decreased growth cone microtubule dynamics and a decreased neurite outgrowth rate of 22% in vitro and in vivo. The effect of the 22% slower growth rate is exacerbated for sensory recovery, where fibers must reinnervate the full volume of the skin to recover touch function. Overall, these data reveal that, while TUBB3 is not required for formation of the nervous system, it has a specific role in the rate of peripheral axon regeneration that cannot be replaced by other β-tubulins. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Alban Latremoliere, Long Cheng, Michelle DeLisle, Chen Wu, Sheena Chew, Elizabeth B Hutchinson, Andrew Sheridan, Chloe Alexandre, Frederic Latremoliere, Shu-Hsien Sheu, Sara Golidy, Takao Omura, Eric A Huebner, Yanjie Fan, Mary C Whitman, Elaine Nguyen, Crystal Hermawan, Carlo Pierpaoli, Max A Tischfield, Clifford J Woolf, Elizabeth C Engle. Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration. Cell reports. 2018 Aug 14;24(7):1865-1879.e9

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 30110642

View Full Text