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RING and U-box ubiquitin ligases promote ubiquitin (Ub) transfer by priming Ub-conjugated E2 in a closed conformation to optimize the thioester bond for nucleophilic attack by substrate lysine. Here, we describe a single-turnover lysine discharge assay for direct assessment of the activity of any RING/U-box E3-E2~Ub complex.


Lori Buetow, Mads Gabrielsen, Danny T Huang. Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays. Methods in molecular biology (Clifton, N.J.). 2018;1844:19-31

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PMID: 30242700

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