Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes.

The recent resolution revolution in cryo-electron microscopy has generated a huge interest in the technique for determining atomic resolution structures of large and dynamic macromolecular complexes that are intractable to crystallography and NMR. A key element of success in cryo-EM is the quality of the specimen vitrified on the cryo-EM grid. In this chapter we outline methods for cryo-EM grid sample preparation.

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Leifu Chang, David Barford. Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes. Methods in molecular biology (Clifton, N.J.). 2018;1844:209-215

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PMID: 30242712

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