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    Physical and chemical methods for nanoparticle synthesis are disadvantageous to less energy demanding and more efficient and environmentally friendly biological approaches. Thus, in this paper, we designed simple, bottom-up, in vitro, static experiment under laboratory conditions using suspension of mixed flower pollen grains for nanoparticle synthesis. Pollen grains provided template substrates for gold nanoparticles synthesis from dissolved Au(III). Transmission and scanning electron microscopy along with ultraviolet-visible spectra confirmed the gold nanoparticles formation. The biosynthesized/phytosynthesized gold nanoparticles had relative narrow size distribution (from 3 to 11 nm) with dominant spherical morphology with no aggregated forms. Thus, the gold nanoparticles in pollen dispersion provides excellent stability and dispersity.

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    Marek Kolenčík, Peter Štrba, Martin Šebesta, Gabriela Kratošová, Martin Urík, Ivo Vávra, Pavlína Peikertová, Nora Polláková, Vladimír Šimanský, Huan Feng, Yu Qian, Illa Ramakanth. Nanogold Biosynthesis Mediated by Mixed Flower Pollen Grains. Journal of nanoscience and nanotechnology. 2019 May 01;19(5):2983-2988

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    PMID: 30501809

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