Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films Under Flow Conditions.

Endothelial cell culture under flow, to mimic physiological conditions within blood vessels, has gained particular attention for the formation of a homogeneous endothelium in vitro. Here, we report on the design of a setup for simultaneous culture of up to nine electrospun membranes or thin polymer films in custom-made holders under flow on an orbital shaker. The versatile design of the device allows for the use of electrospun membranes/polymer films of choice and subsequent analysis with commonly used methods such as immunofluorescence or scanning electron microscopy.

Citation

Anne-Sophie Mertgen, Markus Rottmar, Lukas Weidenbacher, Anne Géraldine Guex. Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films Under Flow Conditions. Methods in molecular biology (Clifton, N.J.). 2020;2125:1-13

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PMID: 30539347

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