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Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essential for yeast cell growth and for the specific accumulation of box C/D snoRNAs. In this work, chromatin, RNA, and protein immunoprecipitation assays revealed the ordered loading of several snoRNP-related proteins on immature and mature snoRNA species. Our results identify Bcd1p as an assembly factor of C/D snoRNP biogenesis that is likely recruited cotranscriptionally and that directs the loading of the core protein Nop58 on RNA. © 2019 Paul et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.


Arnaud Paul, Decebal Tiotiu, Benoît Bragantini, Hélène Marty, Bruno Charpentier, Séverine Massenet, Stéphane Labialle. Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis. RNA (New York, N.Y.). 2019 Apr;25(4):496-506

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PMID: 30700579

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