How a circularized tmRNA moves through the ribosome.

Christopher D Rae, Yuliya Gordiyenko, V Ramakrishnan

Science (New York, N.Y.) 2019 Feb 15

filter terms:

During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA. Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Citation

Christopher D Rae, Yuliya Gordiyenko, V Ramakrishnan. How a circularized tmRNA moves through the ribosome. Science (New York, N.Y.). 2019 Feb 15;363(6428):740-744