Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis.

Anna V Nielsen, Adam T Woolley

Methods in molecular biology (Clifton, N.J.) 2019

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An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.

Citation

Anna V Nielsen, Adam T Woolley. Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis. Methods in molecular biology (Clifton, N.J.). 2019;1972:175-184