Alisol A 24-Acetate Isolated from the Alismatis Rhizoma Improves Hepatic Lipid Deposition in Hyperlipidemic Mice by ABCA1/ABCG1 Pathway.

Xuan Zhou, Qi Ren, Bing Wang, Ge Fang, Yunzhi Ling, Xiantao Li

Journal of nanoscience and nanotechnology 2019 Sep 01

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The Alisol A 24-acetate is an effective component of the Alismatis Rhizoma (AR) extract, which is often used in the treatment of hyperlipidemia. This study explored the effect and mechanism of the Alisol A 24-acetate from AR on lipid deposition in the liver of hyperlipidemic mice. After establishing hyperlipidemic mouse model (Model) by oral high-fat diet (HFD), the animals were treated with Alisol A 24-acetate for 4 weeks. The changes of blood lipid in mice were detected by ELISA. Hematoxylin and eosin (H&E) staining was used to evaluate the degree of liver lipid deposition in hyperlipidemic mice. Quantitative reverse transcriptase PCR (RT-qPCR) was used to detect the expression of ABCG1 and ABCA1 mRNA in the liver. The expression of ABCG1 and ABCA1 protein was detected by Western blotting (WB). After 4 weeks of high-fat diet, the levels of TC, TG, and LDL-C in the mouse were increased, and the HDL-C level was decreased. After treatment with Alisol A 24-acetate, the levels of TC, TG, and LDL-C in the blood of hyperlipidemic mouse were significantly reduced, and the level of HDL-C was increased. The results of H&E staining showed that the lipid deposition in the liver of hyperlipidemic mouse was improved after treatment with Alisol A 24-acetate. RT-qPCR and WB analysis documented that ABCG1 and ABCA1 mRNA and protein expression in hyperlipidemic mice were promoted after the Alisol A 24-acetate treatment. In conclusion, Alisol A 24-acetate effectively alleviates the liver lipid deposition in hyperlipidemic mice, and this effect is achieved mostly by promoting the expression of ABCG1 and ABCA1 at the mRNA and protein levels.

Citation

Xuan Zhou, Qi Ren, Bing Wang, Ge Fang, Yunzhi Ling, Xiantao Li. Alisol A 24-Acetate Isolated from the Alismatis Rhizoma Improves Hepatic Lipid Deposition in Hyperlipidemic Mice by ABCA1/ABCG1 Pathway. Journal of nanoscience and nanotechnology. 2019 Sep 01;19(9):5496-5502