A combined in silico and in vitro study on mouse Serpina1a antitrypsin-deficiency mutants.

Reto Eggenschwiler, Atanas Patronov, Jan Hegermann, Mariane Fráguas-Eggenschwiler, Guangming Wu, Leon Cortnumme, Matthias Ochs, Iris Antes, Tobias Cantz

Scientific reports 2019 May 16

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Certain point-mutations in the human SERPINA1-gene can cause severe α1-antitrypsin-deficiency (A1AT-D). Affected individuals can suffer from loss-of-function lung-disease and from gain-of-function liver-disease phenotypes. However, age of onset and severity of clinical appearance is heterogeneous amongst carriers, suggesting involvement of additional genetic and environmental factors. The generation of authentic A1AT-D mouse-models has been hampered by the complexity of the mouse Serpina1-gene locus and a model with concurrent lung and liver-disease is still missing. Here, we investigate point-mutations in the mouse Serpina1a antitrypsin-orthologue, which are homolog-equivalent to ones known to cause severe A1AT-D in human. We combine in silico and in vitro methods and we find that analyzed mutations do introduce potential disease-causing properties into Serpina1a. Finally, we show that introduction of the King's-mutation causes inactivation of neutrophil elastase inhibitory-function in both, mouse and human antitrypsin, while the mouse Z-mutant retains activity. This work paves the path to generation of better A1AT-D mouse-models.

Citation

Reto Eggenschwiler, Atanas Patronov, Jan Hegermann, Mariane Fráguas-Eggenschwiler, Guangming Wu, Leon Cortnumme, Matthias Ochs, Iris Antes, Tobias Cantz. A combined in silico and in vitro study on mouse Serpina1a antitrypsin-deficiency mutants. Scientific reports. 2019 May 16;9(1):7486