Unmodified household coffee maker assisted extraction and purification of anticancer agents from Dillenia indica fruits.

Mohsin Ali Khan, Tanveer Ahamad, Mohammad Saquib, Mohd Kamil Hussain, Mohammad Faheem Khan

Natural product research 2021 Mar

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Bioassay targeted, 80% aqueous ethanol crude extract of the fruits of Dillenia indica Linn, using the unmodified household coffee maker, afforded five compounds, namely betulinic acid (1), rhamnazin (2), dillenetin (3), luteolin-7-O-β-D-glucoside (4) and hypolaetin-8-O-β-D-glucoside (5). The crude extract, fractions and purified compounds were tested against MDA MB-231, A549 and HeLa cancer cell lines by MTT assay, using betulinic acid 1, as a positive control. Compound 3 showed the best activity against A549 (IC50 = 26.60 ± 2.5 µM) and HeLa cancer cell lines (IC50 =19.35 ± 0.9 µM), whereas compound 5 was found to show the best activity against MDA MB-231 (IC50 = 34.62 ± 5.2µM) cancer cell line. These highly potent anticancer compounds obtained from the fruits of D. indica may be suitable for herbal drug development and formulations.

Citation

Mohsin Ali Khan, Tanveer Ahamad, Mohammad Saquib, Mohd Kamil Hussain, Mohammad Faheem Khan. Unmodified household coffee maker assisted extraction and purification of anticancer agents from Dillenia indica fruits. Natural product research. 2021 Mar;35(6):984-987