Correlation Engine 2.0
Clear Search sequence regions

  • antibodies (2)
  • blood (1)
  • CFH (9)
  • dyes (2)
  • human (3)
  • ligand (1)
  • lipoic acid (1)
  • protein human (1)
  • reaction time (1)
  • serum (1)
  • Sizes of these terms reflect their relevance to your search.

    Silver nanoclusters (AgNCs) were investigated as labels for the development of a fluoroimmunoassay for the complement factor H (CFH). The reductive one-pot synthesis of AgNCs using lipoic acid as a ligand was optimized by varying the concentration of NaBH4, the temperature and the reaction time. The average diameter and crystal structure of the AgNCs (which display red fluorescence) were determined by HR-TEM. The silver concentration was quantified by ICP-MS. Labelling of the antibody against CFH with AgNCs was optimized. The antibody was labeled with the AgNCs without compromising the recognition capabilities of the antibody. A competitive fluoroimmunoassay was then developed. Fluorescence is measured at excitation/emission maxima of 430/660 nm. The assay has a 0.4 ng mL-1 detection limit and a linear range that extends from 1.2 to 23 ng mL-1. The results compare favorably with those obtained by a commercial ELISA kit. The method was applied to the determination of CFH in spiked human serum. Graphical abstract Schematic presentation of the method for the determination of complement factor H (CFH) protein in human blood by using a CFH-antibody labelled with fluorescent silver nanoclusters.


    Eva Valencia, María Cruz-Alonso, Lydia Álvarez, Héctor González-Iglesias, Beatriz Fernández, Rosario Pereiro. Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H. Mikrochimica acta. 2019 Jun 11;186(7):429

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 31187271

    View Full Text