Akt plays indispensable roles during the first cell lineage differentiation of mouse.

Songhua Xu, Lili Pang, Yue Liu, Xiuli Lian, Kaien Mo, Ruimin Lv, Huimin Zhu, Chengyu Lv, Jianmin Lin, Jiandong Sun, Lixuan Xu, Shie Wang

Journal of molecular histology 2019 Aug

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The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ERα in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decrease in the development rate of mouse blastocysts, accompanied by a decrease in the expression level of Yap protein. We conclude that Akt may be indispensable for the first cell lineage differentiation of mouse.

Citation

Songhua Xu, Lili Pang, Yue Liu, Xiuli Lian, Kaien Mo, Ruimin Lv, Huimin Zhu, Chengyu Lv, Jianmin Lin, Jiandong Sun, Lixuan Xu, Shie Wang. Akt plays indispensable roles during the first cell lineage differentiation of mouse. Journal of molecular histology. 2019 Aug;50(4):369-374