Scanning X-ray microdiffraction of decellularized pericardium tissue at increasing glucose concentration.

Cinzia Giannini, Alberta Terzi, Luca Fusaro, Teresa Sibillano, Ana Diaz, Martina Ramella, Viviane Lutz-Bueno, Francesca Boccafoschi, Oliver Bunk

Journal of biophotonics 2019 Oct

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Blood glucose supplies energy to cells and is critical for the human brain. Glycation of collagen, the nonenzymatic formation of glucose-bridges, relates to diseases of aging populations and diabetics. This chemical reaction, together with its biomechanical effects, has been well studied employing animal models. However, the direct impact of glycation on collagen nano-structure is largely overlooked, and there is a lack of ex vivo model systems. Here, we present the impact of glucose on collagen nanostructure in a model system based on abundantly available connective tissue of farm animals. By combining ex vivo small and wide-angle X-ray scattering (SAXS/WAXS) imaging, we characterize intra- and inter-molecular parameters of collagen in decellularized bovine pericardium with picometer precision. We observe three distinct regimes according to glucose concentration. Such a study opens new avenues for inspecting the effects of diabetes mellitus on connective tissues and the influence of therapies on the resulting secondary disorders. © 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Cinzia Giannini, Alberta Terzi, Luca Fusaro, Teresa Sibillano, Ana Diaz, Martina Ramella, Viviane Lutz-Bueno, Francesca Boccafoschi, Oliver Bunk. Scanning X-ray microdiffraction of decellularized pericardium tissue at increasing glucose concentration. Journal of biophotonics. 2019 Oct;12(10):e201900106